Figure 3

Subcellular localization of β-Catenin. (a) Western blot analysis of β-Catenin in nuclear and cytoplasmic fractionated extracts from HCT116 cells treated with SR141716 (SR). The histograms depict the densitometric analysis of cytoplasmic and nuclear amounts of β-Catenin normalized versus GAPDH and Lamin A/C respectively and expressed as percentage of control. (b) RT-PCR analysis of β-Catenin in HCT116 cells untreated (−) or treated (+) with SR141716 (SR). In the lower panel the densitometric analysis of β-Catenin mRNA normalized for actin is shown. Data are shown as mean ± SD (unpaired two tailed Student’s t-test *p < 0.05, **p < 0.01). (c) HCT116 cells treated with SR141716 (10 μM) or vehicle (CTRL) were stained with anti β-Catenin antibody (green fluorescence) and anti Lamin A/C antibody (red fluorescence). In right panels orthogonal view of 0.5 μm thickness in the z plane was reported. Cropped images from full-length gels are displayed in a and b.