Figure 7
Super-resolution imaging microscopy validation of novel PSD-enriched proteins, PLEKHA5 and ADGRA1. (A,E) SIM imaging of primary cultured hippocampal neurons at DIV19 for PLEKHA5 and ADGRA1 (green) along with HOMER1 (red), the latter as a marker for the PSD. Lower panel shows zoom in of the marked area (scale bar 2 µm). (B,C) Line scan analysis on inset from panel a,e shows co-localization between the three proteins at postsynaptic sites. (D) Bar graph showing percentage (mean ± sem) of postsynaptic density HOMER1 puncta positive for PLEKHA5 (dark green, 48.61 ± 4.92, n = 10), ADGRA1 (light green 96.54 ± 1.02, n = 7).
