Figure 2

SDS-PAGE analysis of Vip3 proteins expressed and purified from Pseudomonas. The N-terminal sequence of bands A and C was ¹⁰ANMNN (amino acid #2 by Vip3Ab1 numbering) and for bands B and D was ²VQKWM. Vip3_BA preparations also contained a partially processed form of the protein with an N-terminus of ¹⁹⁰NEKFD (E).