Figure 6

Time course of Vip3_BA digestion by H. zea gut enzymes. Vip3_BA was incubated with H. zea gut enzymes at 23 °C and injected onto an analytical SEC column after different time intervals. Protein peak areas at 10.1 minutes and 13.1 minutes, consistent with monomeric and tetrameric species of protein, were compared. The monomeric protein peak was nearly completely degraded after 45 minutes (solid line, closed circles) whereas tetrameric species (dotted line, open circles) were stable for a longer period of time.