Table 1 Sequences of the qRT-PCR primers.
Gene | Genbank accession | Forward primer | Reverse primer |
|---|---|---|---|
Acta2 | NM_007392.3 | AAGAGCATCCGACACTGCTGAC | AGCACAGCCTGAATAGCCACATAC |
COL1A1 | NM_007742.4 | CAGGGTATTGCTGGACAACGTG | GGACCTTGTTTGCCAGGTTCA |
IFN-γ | NM_008337.4 | TAGCCAAGACTGTGATTGCGG | AGACATCTCCTCCCATCAGCAG |
TNF-α | NM_013963.3 | AAGCCTGTAGCCCACGTCGTA | AGGTACAACCCATCGGCTGG |
IL-6 | NM_031168.2 | CCACTTCACAAGTCGGAGGCTTA | CCAGTTTGGTAGCATCCATCATTTC |
IL-4 | NM_021283.2 | ACAGGAGAAGGGACGCCAT | GAAGCCCTACAGACGAGCTCA |
IL-5 | NM_010558.1 | TGGGGGTACTGTGGAAATGC | CCACACTTCTCTTTTTGGCGG |
IL-10 | NM_010548.2 | GCCAGAGCCACATGCTCCTA | GATAAGGCTTGGCAACCCAAGTAA |
IL-13 | NM_008355.3 | CCCTGGATTCCCTGACCAAC | GGAGGCTGGAGACCGTAGT |
IL-17A | NM_010552.3 | ACCGCAATGAAGACCCTGAT | TCCCTCCGCATTGACACA |
TGF-β1 | NM_011577.2 | GTGTGGAGCAACATGTGGAACTCTA | CGCTGAATCGAAAGCCCTGTA |
FGL2 | NM_008013.4 | TGGACAACAAAGTGGCAAATCT | TGGAACACTTGCCATCCAAA |
GAPDH | NM_001289726.1 | CATGGCCTTCCGTGTTCCTA | CCTGCTTCACCACCTTCTTGAT |
