Figure 2

α-syn is arginylated in vivo. Top, mass spectra of α-syn peptides arginylated in the mouse brain with the full α-syn sequence underneath. The arginylated residues identified in the native mouse brain are indicated in red enlarged font. Bottom, 2D gel comparison of α-syn preparations arginylated in vitro using [14C-Arg] (visualized by Coomassie staining, left, and autoradiography, middle) and native preparation of α-syn from the mouse brain (visualized by Coomassie staining, right). Single and double arrows indicate the spots containing arginylated α-syn.