Figure 1
Expression pattern of Mllt10 mRNA in mouse embryos. Expression of Mllt10 mRNA was detected by whole-mount in situ hybridization of E9.5 (a), E10.5 (b) and E11.5 (d). (c) Non-specific signal detected by sense probe is shown. The low signal suggests specificity of Mllt10 antisense probe in other panels. In each case 3–7 embryos were analyzed. Scale bars; 1 mm. Frontal section of E10.0 embryo stained with antisense probe for Mllt10 at the level of the nasal process (e), and the level of the mandibular and maxillary process (f) are shown. Section images are representative of 2 embryonic specimens analyzed. Scale bars; 100 μm. fl; forelimb bud, fp; frontonasal process, md; mandibular arch, mx; maxillary process, np; nasal process, nt; neural tube, px; pharynx. (g) Expression level of Mllt10 was quantified by RT-qPCR. Quantities of Mllt10 mRNA were normalized to Gapdh mRNA and the ΔCT (CT value of the samples normalized to that of Gapdh) are presented as bar graph. Data are mean ± s.e. of 5 independent experiments. Statistical differences were assessed with Student’s t-test, and p-values are shown.
