Figure 3

Proliferation defects in mesenchymal cells of Mllt10-KO embryo nasal processes. BrdU-positive proliferating cells in nasal process were detected in frontal sections of wild-type (a–f) and Mllt10-KO (g–l) embryo heads at indicated embryonic stages. Mllt10-KO embryos had a marked reduction of BrdU-positive cells at E10.5, indicated by arrowheads in (j). Scale bars; 100 μm. Directional planes for (a–l) were shown in (b) (D; dorsal, V; ventral, M; medial and L; lateral). Bar graph depicting average of numbers of BrdU-positive cells (m), and pH3-positive cells (n) in the nasal processes of E10.0, E10.5 and E11.0 wild-type (WT) and Mllt10-KO (KO) embryos. The number of BrdU-positive cells and pH3-positive cells were counted in both of medial and lateral nasal process on 3 histological sections obtained from 3 different embryos. Data are mean ± s.e. Statistical differences were assessed with Student’s t-test, and p-values are shown. (o–r) BrdU-positive proliferating cells in TIMNPs were detected using frontal sections of E11.0 wild-type and Mllt10-KO embryos. TIMNPs indicated by white dotted-lines. The angle made by opposing medial nasal processes indicated by yellow line. Scale bars; 100 μm. (s) The number of BrdU-positive cells was counted in TIMNPs on 3–4 histological sections obtained from 3 embryos. Data are mean ± s.e. Statistical differences were assessed with Student’s t-test, and p-values are shown.