Figure 4
Mllt10-KO embryos exhibit abnormal forebrain development. (a) Bar graph depicting the percentage of pH3-positive cells in the forebrain of E12.5 wild-type (WT) and Mllt10-KO (KO) embryos. Neuron-specific β-III tubulin was immunostained in frontal sections of E12.5 wild-type (b,c) and Mllt10-KO (d,e) embryonic heads at eye level. The forebrain expanded laterally in Mllt10-KO embryos. Arrowheads indicate the boundary between lateral and medial ganglionic eminences. The boundary was obscure in Mllt10-KO forebrain (asterisks). MGE; medial ganglionic eminence. Scale bars; 500 μm. (f) Bar graph depicting relative area of immunostaining of anti-β-III tubulin antibody in the forebrain of E12.5 wild-type (WT) and Mllt10-KO (KO) embryos. The area of β-III tubulin-positive and the number of pH3-positive cells were analyzed in the forebrain on 3 histological sections obtained from 2 different embryos. Data are mean ± s.e. Statistical differences were assessed with Student’s t-test, and p-values are shown.
