Figure 6
Loss of Mllt10 causes down-regulation of AP2α. Expression of AP2α mRNA was detected by whole-mount in situ hybridization wild-type embryos (a–d) and Mllt10-KO embryos (e–h) at E10.5. Lateral views of whole-embryos (a and e; scale bars 500 μm), lateral views of heads (b and f; scale bars 100 μm), ventral views of heads (c and g; scale bars 100 μm), and frontal sections of nasal process (d and h; scale bars 100 μm) are shown. The decrease of AP2α mRNA expression in the nasal processes and the medial region between processes are indicated by arrowheads and arrow, respectively. Over 10 embryos were analyzed. ec; ectoderm. Directional planes for d and h were shown in d (D; dorsal, V; ventral, M; medial and L; lateral). (i) Expression level of AP2α was quantified by RT-qPCR. Quantities of AP2α mRNA were normalized to Gapdh mRNA and the relative values are presented as bar graph. WT; wild-type, KO; Mllt10-KO. Data are mean ± s.e. of 4 independent experiments. Statistical differences were assessed with Student’s t-test, and p-values are shown.
