Figure 7
Within nasal processes the AP2α promoter, enhancer and coding regions are enriched for Af10-dependent H3K79me2. (a) Schematic representation of the mouse AP2α locus. The position of qPCR amplicons is indicated. Black box; exon, white box; intron, gray box; untranslated region. (b) Chromatin prepared from wild-type (WT) or Mllt10-KO (KO) nasal processes was immunoprecipitated using anti-H3K79me2 antibody (H3K79me2) or control rabbit IgG (IgG). Precipitated DNA was subjected into qPCR. Bar graph shows recovery, expressed as percentage of input. Data are obtained from 4 independent experiments. Data are mean ± s.e. Statistical differences were assessed with Student’s t-test, and p-values are shown. GD; gene desert, ActB; β-actin.
