Figure 6

Improved memory in mice treated with LPC DHA compared to control or free DHA treated mice. In study 1 (A and B), Morris water maze tests of mice treated with vehicle (control), free DHA, sn-1 DHA LPC, and sn-2 DHA LPC for 1 month are shown. LPC DHA-treated mice did not differ from control or free DHA mice in the acquisition phase (A). In the probe trial (B) both sn-1 DHA LPC and sn-2 DHA treated mice reached the previous platform area with shorter latency time, and spent longer in the target quadrant. Data expressed as mean ± S.D (n = 8). *p < 0.05, by two-way ANOVA followed by Tukey’s post hoc comparisons. In a second study (C and D) to validate the above results, only 2 groups of mice were used and were treated either with free DHA or sn-1 DHA LPC for 1 month. As in the first study, sn-1 DHA LPC treated mice reached the previous platform area with a lower latency time, and spent longer in the target quadrant, compared to free DHA group (n = 5 each). Data are expressed as mean ± S.D. *p < 0.05 by two-way ANOVA followed by Tukey’s post hoc comparisons.