Figure 1 | Scientific Reports

Figure 1

From: Hypoxia-induced ANGPTL4 sustains tumour growth and anoikis resistance through different mechanisms in scirrhous gastric cancer cell lines

Figure 1

Analysis of ANGPTL4 expression in nine gastric cancer (GC) cell lines cultured under normoxia and hypoxia for 24 h. (a) RT-qPCR analysis of ANGPTL4 expression in nine GC cell lines. Relative expression of ANGPTL4 mRNA was determined as the expression ratio of ANGPTL4 mRNA/β-actin mRNA. The experiments were performed in triplicate and repeated three times. The data are presented as the mean ± SD. P values < 0.05 indicate a significant difference, as marked by an asterisk. (b) Western blot (WB) analysis of ANGPTL4 expression in nine GC cell lines. Strong ANGPTL4 expression (upper panel) was induced in 58As9 and 44As3 cells after 24 h of hypoxia. The internal marker β-actin is shown in the lower panel. The experiments were repeated three times. N, normoxia; H, hypoxia.

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