Figure 3 | Scientific Reports

Figure 3

From: Hypoxia-induced ANGPTL4 sustains tumour growth and anoikis resistance through different mechanisms in scirrhous gastric cancer cell lines

Figure 3

Establishment of stable ANGPTL4-KD clones of 58As9 and 44As3 cells. (a) WB analysis of the effect of ANGPTL4 KD on the 58As9 or 44As3 transfectant (upper panel). SC: negative scramble siRNA transfectant, KD: ANGPTL4 siRNA transfectant. The expression of internal control β-actin is shown in the lower panel. The experiments were repeated three times. (b) The concentration of secreted ANGPTL4 was estimated using an ELISA (58As9, left; 44As3, right). The experiments were performed in triplicate and repeated three times. The data are presented as the mean ± SD.

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