Figure 2 | Scientific Reports

Figure 2

From: Altered methylations of H19, Snrpn, Mest and Peg3 are reversible by developmental reprogramming in kidney tissue of ICSI-derived mice

Figure 2

DNA methylation of the H19 and Snrpn DMRs in kidney tissues of adult and old ICSI-derived and TCET conceived mice. (a) Schematic representation of the differential methylated regions analyzed by bisulfite sequencing PCR (BSP) or pyrosequencing or both of them. The target sequences examined by pyrosequencing were in the boxes and CpG sites were underlined. (b) Methylation profiles by BSP (12 CpG sites) for the H19 DMR from ICSI-derived and TCET conceived mice. (c) Methylation profiles by BSP (16 CpG sites) for the Snrpn DMR from ICSI-derived and TCET conceived mice. (d) Statistical methylation analysis of H19 DMR and Snrpn DMR (Pearson chi-square test was used for the analysis: **p < 0.01; *p < 0.05).

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