Table 2 Primer sequences used for bisulfite pyrosequencing.

H19 DMR island3

(Forward) 5′-TTTTTGGGTAGTTTTTTTAGTTTTG-3′

211

(chr7:142580208-142580418)

(Reverse) 5′-biotin-ACACAAATACCTAATCCCTTTATTAAAC-3′

Snrpn DMR

(Forward) 5′-TTTTGGTAGTTGTTTTTTGGTAGG-3′

240

(chr7: 60005020-60005259)

(Reverse) 5′-biotin-CACAAACCCAACTAACCTTCC-3′

Mest DMR

(Forward) 5′-TTTAGGGTGTTTGTATTGTGATTG-3′

181

(chr6: 30737310-30737490)

(Reverse) 5′-biotin-TCACATAAAATAAACCAAAATCACC-3′

Peg3 DMR

(Forward) 5′-ACCAACCCAAAATAAACATCTCT-3′

115

(chr7: 6730345-6730459)

(Reverse) 5′-biotin-AGAGGATTTTGATAAGGAGGTGT-3′