Table 3 Reverse primers for real-time RT-PCR.
Gene (GenBank accession) | Primer Sequences (from 5′ to 3′) | Product size (bp) |
|---|---|---|
H19 | (Forward) 5′-GCACTAAGTCGATTGCACTGG-3′ | 163 |
(NR_001592.1) | (Reverse) 5′-AGGTGCCTGCATCAAGGTGAC-3′ | |
Snrpn | (Forward) 5′-GGATTAGCAGGCCCTGTCAGA-3′ | 166 |
(NM_013670.3) | (Reverse) 5′-TGCCTACAGGTGGAGGTGGA-3′ | |
Mest | (Forward) 5′-GTGGTCGGAAGCCCTGAGATAG-3′ | 112 |
(NM_008590.2) | (Reverse) 5′-GGGCGATCACTCGATGGAA-3′ | |
Peg3 | (Forward) 5′-AAGCCCTTGGGTGTGAGCA-3′ | 131 |
(NM_008817.2) | (Reverse) 5′-CCACTTCGGCTCATGTCGTC-3′ | |
Gapdh | (Forward) 5′-TGTGTCCGTCGTGGATCTGA-3′ | 150 |
(NM_008084.3) | (Reverse) 5′-TTGCTGTTGAAGTCGCAGGAG-3′ |
