Figure 2 | Scientific Reports

Figure 2

From: Interrogation of γ-tubulin alleles using high-resolution fitness measurements reveals a distinct cytoplasmic function in spindle alignment

Figure 2

Identification of synthetic genetic interactions based on relative fitness using GAMER. (a) Overview of the GAMER method, which includes imaging every two hours over a 72-hour period. Images are processed computationally and fitness extracted and used to calculate the GAMER scores of the double mutants. (b,c) SGA growth curves (left panels) for six isolates of wild-type, γtub-Y445D (top) or γtub-Y362E (bottom), mad2Δ and the combined double mutant. Values of the observed and expected growth defects (dij) for the respective double mutants (right panels).

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