Figure 6
Identification of gin-encoded genes required for gerE rearrangement. (a) Schematic of the deletion series of the gin element. The deleted regions within the gin element in B. subtilis 168Gin are shown. Arrows indicate the PCR primers used to detect gerE rearrangement. The location of the putative RDF for GirC was inferred as the shaded area. (b) Detection of gerE rearrangement. PCR was performed to detect the composite gerE (925 bp) after rearrangement in 168Gin, GCd, GABd, and GR1–3 strains using chromosomal DNA from cells at the vegetative (T−1) and sporulation (T6) phases. (c) Conservation of girX in the gin elements in B. cereus and B. toyonensis strains. The girC and girX genes are colored red and orange, respectively. (d) Alignment of the amino acids sequences of GirX from B. cereus strains ATCC10987, AH820, and Q1, and from B. toyonensis BCT-7112. The amino acids conserved in three out of the four and in all four are shown in grey and black, respectively. (e) Complementation test for GirX. The girX gene was introduced into the GR2 strain (GR2X). PCR was performed to detect the composite gerE after the rearrangement under the same conditions as shown in (b). The original agarose gel images of Fig. 6 are presented in Supplementary Figure S7.
