Figure 3
From: Oleic acid induces apoptosis and autophagy in the treatment of Tongue Squamous cell carcinomas
OA induces apoptosis in TSCC cells. The TSCC cells were treated with different concentrations of OA (0, 50, 100, or 150 μM) for 24 h. (a) Flow cytometric plots showing OA-induced apoptosis in CAL27 and UM1 cells. The cells in the lower right corner represent early apoptosis, and the top right corner shows the dead cells. (b) Bar graphs showing that 24 h OA treatment induced a marked apoptotic cell death in CAL27 and UM1 cells. Data are the mean ± standard deviation of three independent experiments (**p < 0.01, ***p < 0.001). (c) TUNEL assay showing OA-induced apoptosis of CAL27 and UM1 cells after treatment with OA. Arrows indicate apoptotic cells. (d) The expression of apoptosis-related proteins (Bcl-2, caspase-3, cleaved-caspase-3 and p53) was detected by Western blot. GAPDH was used as the internal control.
