Figure 4
From: Oleic acid induces apoptosis and autophagy in the treatment of Tongue Squamous cell carcinomas
OA induces autophagy in TSCC cells. (a) OA-induced autolysosome formation in CAL27 and UM1 cells was observed under a TEM after the TSCC cells were treated with different concentrations of OA (0 or 150 μM) for 24 h. Arrows indicate autolysosomes. (b,c) Western blot displaying the expression levels of LC3, p62 in TSCC cells after treatment with OA for 24 h. GAPDH was used as the internal control. (d,e) LC3 protein levels from the above Western blot were quantitated, and the ratio between LC3 I (16 kDa) and LC3 II (14 kDa) was calculated. OA induced conversion of the LC3 protein from LC3 I to LC3 II. Bar graphs showing the LC3 I/LC3 II ratio was significantly decreased after treatment with OA. Data are the mean ± standard deviation of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001).
