Figure 1
From: The gibberellin GID1-DELLA signalling module exists in evolutionarily ancient conifers
Multiple sequence alignment of protein sequences encoded by PtGID1-like genes and A. thaliana and O. sativa GID1 genes. Black and gray boxes indicate identical or similar residues. The 17 arrows at the top indicate the residues essential for the gibberellic acid binding activity of GID1 in angiosperms. The two asterisks at the bottom indicate sites that are not identical for PtGID1-like genes within the conserved sequence region found in angiosperms. The triangle indicates similar residues in AtGID1b, AtGID1c, and PtGID1, and in OsGID1 and AtGID1a. As in rice and Arabidopsis, three conserved amino acids (S, D, and H) shape the catalytic triad in the HSL family (Nakajima et al.16; Ueguchi-Tanaka et al.8). Two of them (S and D) are conserved in PtGID1, while the third (H) is replaced by V or I. There are 13 functional domains (TWVLIS, LDR, FFHGGSF, HS, IYD, YRR, DGW, GDSSGGNI, GNI, MF, LDGKYF, WYW, and GFY) in GID1 (Hirano et al.14). Within TWVLIS, W is replaced by F, while all other domains are conserved. We confirmed the presence of all amino acid residues essential for GA binding encoded within the cloned PtGID1 gene.
