Figure 8
From: The gibberellin GID1-DELLA signalling module exists in evolutionarily ancient conifers
BiFC analysis of the GID1-DELLA interaction in nuclei of transfected A. thaliana. AtGID1 and PtGID1 were expressed and interactions tested with wild-type and mutant A. thaliana and P. tabuliformis DELLA proteins (AtGAI, Atgai; PtDPL, Ptdpl; PtRGA; Ptrga), respectively. Bright-field image, YFP fluorescence image, and the merged image are each displayed for expression of PtGID1 alone (A); co-expression with PtDPL (B), AtGAI (C), PtRGA (D), Δ17-domain mutant Ptdpl (E), Δ17-domain mutant Atgai (F), and Δ17-domain mutant Ptrga (G); Bright-field image, YFP fluorescence image, and the merged image are each displayed for expression of AtGID1 alone (H); co-expression with PtDPL (I), AtGAI (J), PtRGA (K), Δ17-domain mutant Ptdpl (L), Δ17-domain mutant Atgai (M), and Δ17-domain mutant Ptrga (N).
