Figure 7
From: Berberine ameliorates fatty acid-induced oxidative stress in human hepatoma cells
The change in mitochondrial electron transport chain complex expression and oxygen consumption by BBR. (A) A representative western blot for electron transport chain complex I, II, III, and V in Huh 7 cells treated with FFAs and BBR. The densitometric units of each protein band were calculated from three independent experiments versus the control group. (B) The oxygen consumption rate was determined by Seahorse XF-24 in HepG2 cells treated with FFAs and BBR in response to mitochondrial compounds, including oligomycin, FCCP, antimycin, and rotenone. (C) Oxygen consumption rates of basal respiratory capacity were calculated from the real-time oxygen consumption curve presented in (B). *p < 0.05. Data are expressed as the mean ± SEM. *p < 0.05 vs control, #p < 0.05 vs FFA.
