Figure 3 | Scientific Reports

Figure 3

From: MiR-503 modulates epithelial-mesenchymal transition in silica-induced pulmonary fibrosis by targeting PI3K p85 and is sponged by lncRNA MALAT1

Figure 3

MiR-503 directly targets and down-regulates expression of PI3K p85. (a) Schematic diagram showing miR-503 targeting sequences or mutated sequences from the 3′-UTR of the PI3K p85 gene. (b) The protein levels of p-PI3K p85 and PI3K p85 in mouse lung tissues treated with silica for 7, 14 and 28 days were detected by western blot analysis. And the relative protein levels (means ± SD, n = 3) were analyzed by ImageJ, *P < 0.05 and **P < 0.01 versus the control group. (c) The protein expression of PI3K p85 in mouse lung tissues treated with miR-503 agomir for 28 days were determined by western blot and found decreased compared with the SiO2 + miR-NC agomir group, *P < 0.05 and **P < 0.01 versus the SiO2 + miR-NC agomir group. (d) Effects of miR-503 mimic on PI3K p85 3′-UTR luciferase reporters in HBE cells. Luciferase activities were calculated as the ratio of firefly/renilla activities and normalized to the miR-NC + p85 WT group, **P < 0.01 versus the miR-NC + p85 WT group.

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