Figure 2 | Scientific Reports

Figure 2

From: Inhibition of fucosylation by 2-fluorofucose suppresses human liver cancer HepG2 cell proliferation and migration as well as tumor formation

Figure 2

Comparison of N-glycan profiles in HepG2 cells treated with and without 2FF. (A) MS spectrum of the permethylated glycans from the cells treated with DMSO only (upper) and DMSO containing 2FF (lower). (B) Percentages of fucosylated complex glycans in total complex glycans from HepG2 cells. The values were calculated from peak areas in the range of m/z 1,500-3,500 of the MS spectra. (C) HepG2 cells were cultured with 2FF for 3 days at different concentrations as indicated, and then equal amounts of cell lysates were probed with Con A, which specifically recognizes alpha-linked mannose and glucose; WGA, which selectively binds to N-Acetyl glucosamine (GlcNAc) and SNA, which specifically recognizes α 2, 6 sialylation. GAPDH was used as a loading control.

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