Figure 5
From: The multifunctional polydnavirus TnBVANK1 protein: impact on host apoptotic pathway
TnBVANK1 protein colocalises with Alix in polyclonal S2 cell line. i-iv) Polyclonal S2 cell line coexpressing both TnBVANK1 and Alix proteins stained with anti-TnBVANK1 and anti-Alix antibodies. v-viii) Staining of control S2 cells expressing only Alix protein. The localisation of TnBVANK1 and Alix proteins was determined by FITC (green signal) and TRITC (red signal) fluorescence, respectively and nucleus was stained with DAPI (blue signal). In merged images there is a clear overlap of red and green signals showing a colocalisation (yellow signal) of analysed proteins. Images were observed at immunofluorescence microscopy using Nikon Eclipse 80i equipped with a Nikon Plan Fluor 100x/0.5–1.3 Oil Iris objective, the images were recorded with Nikon Digital Sight DS-U1 camera and ImageJ software.
