Figure 6

Alix and TnBVank1 transcript levels in S2 cells and post-Alix silencing caspase-3 assay. (a) Expression levels of Alix and TnBVank1 at different hours (T₀, T₆, T₁₀) after Alix silencing in S2 cells stably expressing TnBVank1 (S2[ank1] Alix Si), normalised to the endogenous control Rpl32⁵⁴. As control dsApisOBP3 was used (S2[ank1] OBP3 Si). No expression of TnBVank1 was observed in the control S2 cells. (b) Western blot of Alix expression at different hours (T₀, T₆, T₁₀) after Alix silencing in S2 cells stably expressing TnBVank1. Original Western Blot is reported in Supplementary Information. (c) Post-Alix silencing caspase-3 activity in S2 cells, S2 cells stably expressing TnBVank1 (S2[ank1]), S2 cells stably expressing TnBVank1 with silenced Alix transcript (S2[ank1] Alix Si) and S2 cells stably expressing TnBVank1 transfected with dsRNA of ApisOBP3 as control (S2[ank1] OBP3 Si), harvested at different times (0, 6 and 10 h) after the replacement of the medium containing dsRNA. Data represent the mean of 3 replicates ± SD. Statistically significant differences between samples are indicated with asterisk (***P ≤ 0.001, one way ANOVA and Tukey’s Test P < 0.05).