Figure 7

Alix and TnBVank1 transcript levels in H. virescens haemocytes and post-Alix silencing caspase-3 assay. (a) Expression level of Alix and TnBVank1 transcripts into H. virescens haemocytes 56 h after Alix silencing (Hemo[ank1] Alix Si), normalised to the endogenous controls (EF1α and RP13⁴⁹). No expression of TnBVank1 was observed in the control haemocytes transfected with the empty pIZT/V5-His vector (Hemo). (b) Western blot of Alix expression in haemocytes 56 h after Alix silencing and transfected with TnBVank1(Hemo[ank1] Alix Si); as control haemocytes were transfected with the empty vector (Hemo). Original Western Blot is reported in Supplementary Information. (c) Post-Alix silencing caspase-3 activity in haemocytes, transfected with TnBVank1 gene (Hemo[ank1]), cotransfected with Alix dsRNA and TnBVank1 (Hemo[ank1] Alix Si). As control haemocytes were transfected with the empty vector (Hemo) and cotransfected with ApisOBP3 dsRNA and TnBVank1 (Hemo[ank1] OBP3 Si). Data represent the mean of 3 replicates ± SD. Statistically significant differences between samples are indicated with asterisk (***P ≤ 0.001, one way ANOVA and Tukey’s Test P < 0.05).