Figure 3
Rogdi mutation does not dampen sleep rebound after mechanical sleep deprivation. (a) Sleep behaviors in individual flies were monitored in LD cycles at 25 °C. Where indicated, flies were subject to the mechanical stimulus for the first 9 hours in the D phase (from ZT12 to ZT21) and their sleep rebound was quantitatively analyzed for the last 3 hours in the D phase (from ZT21 to ZT24). Averaged sleep profiles on the day of mechanical sleep deprivation (SD) (darker lines) were overlaid by those on the previous day (lighter lines). ZT, zeitgeber time (lights-on at ZT0; lights-off at ZT12). (b) Cumulative sleep loss was calculated in individual flies and averaged per each genotype. To calculate %sleep loss at each time-point, total sleep amount from ZT12 to a given time-point on the day of SD was subtracted by that on the previous day and then normalized to the total sleep amount from ZT12 and ZT21 on the previous day. Data represent average ± SEM (n = 31–40). Two-way repeated-measures ANOVA detected significant effects of genotypes (F[2,100] = 4.707, P = 0.0111) and their interaction with time-points (F[46,2300] = 2.495, P < 0.0001). **P < 0.01, ***P < 0.001 to control at each time-point as determined by Dunnett post hoc test.
