Figure 3

Functional CD4⁺ and CD8⁺ T-cell responses to CMV and HIV antigens. PBMCs were stimulated with different CMV antigenic preparation and the HIV p24 recombinant protein. The specific CD4⁺ and CD8⁺ T-cell responses were measured by flow cytometry in CMV⁺ HIV-uninfected individuals (blue bars, n = 12), CMV⁺ immunoconcordant individuals (green bars, n = 17 and n = 6 for CMV antigens and p24, respectively) and CMV⁺ immunodiscordant individuals (red bars, n = 12 and n = 8 for CMV antigens and p24, respectively). Response magnitudes are reported as the percentages of CD4⁺ and CD8⁺ T-cells producing IFN-γ and IL-2 after background subtraction. Bars indicate mean values ± SEM. Individual data of all subjects are represented by dots. Differences were tested using Mann-Whitney U nonparametric test (*p < 0.05).