Figure 5 | Scientific Reports

Figure 5

From: In vivo target exploration of apidaecin based on Acquired Resistance induced by Gene Overexpression (ARGO assay)

Figure 5

(a) In vitro inhibitory assay of lacZ expression with different stop codons. Lanes 1 and 2; lacZ-UAA, lanes 3 and 4; lacZ-UGA, and lanes 5 and 6; lacZ-UAG. Lanes 2, 4, and 6; 200 μM apidaecin added. (b) In vitro inhibitory assay of lacZ (UAG) expression by apidaecin with modified concentrations of release factors. PrfB was omitted in all conditions. Lanes 1 and 2; 100 nM PrfA, lanes 3 and 4; 20 nM PrfA. Lanes 1 and 3; no apidaecin, lanes 2 and 4; 200 μM apidaecin.

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