Table 1 Limiting dilution assay for CD24, CD44, EpCAM and EpCAM/CD44 cells from xeno-B110 (first generation).
| Â | Cell inoculation number | Number of tumours/Number of inoculated mice | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
H2Kd neg | CD24 br | CD24 dim | CD44 br | CD44 dim | EpCAM br | EpCAM dim | EpCAM/CD44dbr | EpCAM/CD44ddim | ||
xeno-B110 | 30,000 | 4/4 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 |
10,000 | 4/4 | 6/6 | 6/6 | 5/5 | 5/5 | 5/5 | 5/5 | 6/6 | 6/6 | |
5,000 | 5/6 | 5/5 | 6/6 | 4/4 | 4/4 | 6/6 | 6/6 | 5/5 | 5/5 | |
2,000 | ND | 5/5 | 4/4 | 5/5 | 3/5 | 5/5 | 4/5 | 5/5 | 5/5 | |
500 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 2/3 | 3/3 | 2/3 | |
100 | 2/6 | 2/6 | 1/6 | 5/6 | 1/6 | 3/6 | 2/6 | 5/6 | 1/6 | |
10 | 0/6 | 1/6 | 0/6 | 2/6 | 2/6 | 0/6 | 0/6 | 1/6 | 0/6 | |
Estimated TIC frequency (95% CI) |  | 1 in 1177 (393–3526) | 1 in 159 (63–404) | 1 in 285 (108–754) | 1 in 45 (18–110) | 1 in 787 (333–1859) | 1 in 147 (58–373) | 1 in 730 (308–1728) | 1 in 56 (23–135) | 1 in 462 (181–1181) |
p value | Â | Â | 0.42 | <0.001 | 0.01 | <0.01 | ||||
Enrichment factor (br/dim) | Â | Â | 1.79 | 17.49 | 4.97 | 8.25 | ||||
