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Figure 1

From: RETRACTED ARTICLE: The melanocortin signaling cAMP axis accelerates repair and reduces mutagenesis of platinum-induced DNA damage

Figure 1

cAMP signaling enhances the repair of platinum-induced DNA damage. HEK293 cells were pre-treated with either forskolin (10 µM), or vehicle (1% EtOH) for 30 minutes before (A) cisplatin, (B) carboplatin, (C) oxaliplatin, (D) phenanthriplatin treatment (100 µM each) for 1 hr. DNA repair was determined at 3 and 9 hr post-damage by measuring amplification by XL-PCR of a 5 kb fragment of the HPRT gene. Efficiency of PCR amplification between damage and control samples was used to calculate repair at each time point. Repair levels in forskolin treated cells significantly different from vehicle at indicated time points were determined by 2-way ANOVA (*p ≤ 0.05). Data are expressed as mean ± SEM from three independent experiments.

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