Figure 2 | Scientific Reports

Figure 2

From: RETRACTED ARTICLE: The melanocortin signaling cAMP axis accelerates repair and reduces mutagenesis of platinum-induced DNA damage

Figure 2

MC1R-cAMP signaling enhances chromatin-bound protein levels of ATR, XPA and AKAP12 following cisplatin treatment. (A) Wild-type primary human melanocytes (PHM). (B) MC1R WT-expressing HEK293 cells or (C) MC1R R151C-expressing HEK293 cells were pre-treated with either forskolin (10 µM), MSH (100 nM), MSH (100 nM) + ASIP (100 nM) or MSH (100 nM) + HBD3 (100 nM) 30 minutes before cisplatin (100 µM) or mock treatment for 1 hr. Chromatin extracts were isolated and probed for either anti-XPA, anti-ATR, anti-AKAP12 or anti-H2A by Western blotting. ATR and XPA bands were cropped from the same blot. AKAP12 bands were cropped from a separate blot.

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