Figure 3 | Scientific Reports

Figure 3

From: RETRACTED ARTICLE: The melanocortin signaling cAMP axis accelerates repair and reduces mutagenesis of platinum-induced DNA damage

Figure 3

MC1R-cAMP signaling enhances interactions between XPA, ATR-pS435 and AKAP12 to a cisplatin-damaged substrate by ORiP. MC1R WT- or MC1R R151C-expressing HEK293 cells were pre-treated with either forskolin (10 µM), MSH (100 nM), MSH (100 nM) + ASIP (100 nM) or MSH (100 nM) + HBD3 (100 nM) 30 minutes before cisplatin treatment (100 µM) for 1 hr. Isolated nuclear extracts were incubated with a cisplatin-damaged DNA fragment (which acts as a substrate for NER) for a period of 30 minutes at 30 °C, as described in the Experimental Procedures. The interaction with the DNA substrate with (A) XPA, (B) ATR-pS435, (C) AKAP12 were determined using antibodies and quantified as described in Experimental Procedures for ORiP. Data are expressed as mean ± SEM from three independent experiments.

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