Figure 6

Immunohistochemical analysis to identify the localisation of RAGE expression in the lung alveoli. The red signal indicates the expression of RAGE. The green signal indicates the expression of RECA1-positive cells (alveolar endothelial cells), podoplanin-positive cells (ATI cells), p180 lamellar body protein-positive cells (ATII cells) and CD68-positive cells (alveolar macrophages), respectively. (A) The white dashed square shows the region from which the higher magnification image was taken. The white arrowheads in the respective panels indicate enhanced expression of RAGE in response to crush injury in endothelial cells and ATI cells. Expressions of RAGE were not enhanced on the ATII cells and alveolar macrophages. Bars represent 50 µm in the top and middle panels (original magnification ×400) and 10 µm in the bottom panels (original magnification ×2000). (B,C) The images in the lateral (X-Y) and axial (Z) direction are shown. The white arrowheads in the respective panels indicate the co-localization between RAGE and alveolar endothelial cells (B) and ATI cells at the plasma membrane (C). XY, YZ and XZ indicate the X-Y, Y-Z and X-Z images respectively. Bars represent 10 µm in the panels (original magnification ×1000).