Figure 3
Kinetic high depth screen of kinase inhibitors. (a) Example rocket plots of five representative cells over six conditions. Each horizontal line represents a single cell over time, where thickness correlates with microns moved since previous hour, colorimetric represents confidence of cell state based on morphology, and horizontal bars represent cell division or death. (b) Heatmap depicting clustering of compounds by four super-metrics derived from rocket plots, normalized to the mean value of all conditions (see Methods). Targeted BRAF compounds (green), MEK inhibitors (orange), Bcr-Abl (red) and cell cycle inhibitors (yellow) are highlighted. Compounds dissolved in DMSO are underlined. Based on observation of 775 cells across 17 different treatment conditions. (c) Average accuracy of control conditions (Staurosporine, PD0332991, and not treated) at indicated time points. Accuracy peaks at 24 hours (the time-point the experiments used to train the classifier were captured) and drops after 40 hours.
