Figure 1
Characterisation of efnA2 KO adult neocortex. Adjacent coronal sections of efnA2 KO adult brain stained with the nuclear dye Hoechst (a) and labeled with the neuron-specific transcription factor NeuN (a’), arrowheads demarcate the maximum width for this patch of low NeuN density. efnA2 KO neocortex stained with Hoechst (b,c) and labeled with NeuN (b’, c’) illustrating abnormal neuronal density in layers 2/3 (b, b’) and layer 5 (c, c’). (b”, c”) Linear graph plot of the signal intensity for each stain measured in the boxed area in (b and c) respectively. efnA2 KO neocortex labeled with the astrocytes marker ALDH1L1 (d), the oligodendrocyte marker Olig2 (d’) and the microglia marker Iba1 (d”), hatched lines delineate regions exhibiting abnormal NeuN density ((d–d”) inset). Quantification of NeuN+ cells density in wild type (e) and efnA2 KO (e’) in neocortical regions exhibiting normal laminar architecture ((e”) error bars represent SEM; hatched line in (e’) illustrates an example of zone excluded from the counting frame) in was quantified across all layers by superimposing a grid over the region of interest (schematic in (e)). Scale bars (a’) 250 µm; (b’,c’ and e’) 100 µm; (d”) 50 µm.
