Figure 3
ephrin-A2 reverse signalling inhibits cortical progenitor proliferation. (a) Embryonic neocortex electroporated at E15.5 with GFP-tagged control scrambled shRNA (sh_ctrl-GFP) and GFP-tagged shRNA to knockdown efnA2 (sh_efnA2-GFP) and analyzed 3 days post electroporation (dpe) were labeled with the proliferation marker Ki67 (a’) quantification of the mitotic fraction of electroporated GFP+ cells in the ventricular zone (n = 5; p = 0.01; Mann-Whitney test, error bars represent SEM). (b) Dissociated E14.5 neocortex cultured in presence of BrdU and clustered Fc fragment (control), (b’) ephrin-A2-Fc and (b”) EphA4-Fc, labeled with the neuronal marker TuJ1 (magenta), the thymidine analogue BrdU (green) and counterstained with the nuclear dye Hoechst (white) (b”’) the number of BrdU+ cells was quantified to assess the proliferation after 24 hours in culture. (n = 3; p < 0.001; Kruskal-Wallis test, error bars represent SEM). Scale bar (a) 50 µm; (b”) 100 µm.
