Figure 5
Dramatic reduction of calbindin expression throughout the neocortex of efnA2 KO animals. (a) Hoechst nuclei staining of efnA2 KO neocortex demarcating the laminar architecture of the neocortex, double-labeled with (a’) the neuron-specific transcription factor NeuN, (a”) with the interneuron marker calbindin (Cb). Hatched boxes in (a–a”) delineate the patches of lower neuronal density. (b–b’) Example of Cb staining in the neocortex of adult wild type and efnA2 KO animals, schematic in (b) illustrates the region that was analyzed along the antero-posterior axis of the brain by applying a grid over the zone counted, regions showing abnormal density were demarcated by NeuN double-labeling and excluded. (b”) density of Cb+ interneurons across the neocortical layers of WT and efnA2 KO (n = 4, p < 0.03, Mann-Whitney test, error bars represent SEM); WM white matter; scale bar (a” and b’) 200 µm.
