Figure 7
Loss of ephrin-A2 reduces migration of POA-born interneurons. Coronal slices of brain electroporated at E14.5 with (a) GFP-tagged control scrambled shRNA (sh_ctrl-GFP) or (b) GFP-tagged shRNA to knockdown efnA2 (sh_efnA2-GFP) and cultured for 48 hours (2 days in vitro div) (c) Electroporated cells migration was established by calculating the ratio of GFP+ cells in 100 µm bin (origin = electroporation site) over the total number of GFP+ cells in the section (schematic in (c) see blue dashed lines) revealing a larger fraction of cells closer to the site of electroporation following sh_efnA2-GFP electroporation (n = 15 independent sections from separate embryos analyzed for each condition, p = 0.049; 0.0034, unpaired t test, error bars represent SEM) Scale bar (b) 200 µm.
