Table 2 Oligonucleotides used in this study.

From: Crystal structure of an ASCH protein from Zymomonas mobilis and its ribonuclease activity specific for single-stranded RNA

 

Sequences

ssRNA(30mer)

5′-AUCAGCUCGUCACAACAUUACUUCAUCAAC-3′ (Figs 3a,b and 4d)

ssDNA(30mer)

5′-ATCAGCTCGTCACAACATTACTTCATCAAC-3′ (Figs 3a and 4d)

probe 1, ssRNA(17mer)

5′-CCCGACAACAGGCCCCC-3′ (Fig. 3c)

probe 2, ssRNA(17mer)

5′-CCCCAUAAUAGGCCCCC-3′ (Fig. 3c)

probe 3, ssRNA(17mer)

5′-CCCGACAGCCGGCCCCC-3′ (Fig. 3d)

probe 4, ssRNA(17mer)

5′-CCCGACA(m)GCCGGCCCCC-3′ (2′-Ome, Fig. 3d)

  1. The methylated nucleotide is indicated with “m” in the parenthesis, while the theoretical cleavage sites of ssRNA (17mer)s are indicated with “”.