Figure 1

Schematic depiction of the data acquisition for the SPIM-OPT setup. Starting from the left a tumor spheroid, stained with the DRAQ7^TM fluorescent dye, is inserted into a FEP tube and mounted to the rotation stage. (1) The specimen is rotated at a known angle and then (2) is scanned through the light sheet along the detection axis. The illumination light sheet is established orthogonally to the detection axis and it fulfills the objective focal plane, in order to reduce out of focus contribution during the camera acquisition. While translating the specimen a SPIM detection (2D images stack) is stored (3). Finally (4), the data acquired is used to calculate the Average Intensity Projection (AIP) as a function of the angle. This procedure is repeated by rotating the sample (1) in steps of 2° until performing a complete rotation.