Figure 5

Imaging performance of the PRT technique. Hyperstack projections for the SPIM-OPT measurements (panels A–C) and the PRT reconstructions (panels D–F). The color code denotes the depth at which the fluorophore is located, starting from the first signal. It is important to notice that the two methods share the same coloring order and the same fluorophore distribution, validating our reconstruction. Moreover, PRT shows a fluorophore while SPIM cannot resolve it, due to its location on the other side of the spheroid (red to white in the color scale). The Z hyperstack suggests comparable resolutions between the two methods along the tomographic axis. Finally, panel G locates in space the groups of fluorophores dashed in panel D,E, showing that the small circle includes a cell that belongs as well to the bigger one.