Figure 5 | Scientific Reports

Figure 5

From: Bisphenol A Affects on the Functional Properties and Proteome of Testicular Germ Cells and Spermatogonial Stem Cells in vitro Culture Model

Figure 5

Analysis of meiotic abnormalities during spermatogenesis in recipient mice transplanted with bisphenol A (BPA)-treated cultured germ cells. (A) Representative pachytene spermatocytes of control and 10 µM BPA treatment group. White arrow indicates a synaptonemal complex (SC) lacking an MLH1 focus in 10 µM BPA treatment group. MLH1 = green, SYCP3 = red. (B) The frequency of cells with an SC lacking MLH1 in control and 10 µM BPA treatment group (*compared with control, P < 0.05). (C) Total autosomal SC length (μm) in pachytene cells from control and 10 µM BPA treatment group. (D) Representative zygotene spermatocytes from control and 10 µM BPA treatment group showing RAD51 foci. RAD51 = green, SYCP3 = red. (E) Bar graph represents RAD51 foci count in control and 10 µM BPA treatment group. (F) γH2AX foci formation in pachytene spermatocyte. For both control and BPA 10 µM BPA treatment group, γH2AX foci is localized only in sex body (X and Y chromosomal region), not in autosomes. Scale bars in all pictures = 10 µm.

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