Figure 1

Absorption and emission spectra of the FRET pairs investigated in this study. The spectra were recorded from purified proteins and were normalized to their peak values. Solid lines indicate absorption spectra and dashed lines indicate emission spectra. All lines are colored according to the emission wavelength of the fluorescent protein. All spectra show the donor mTurquoise2 (mTq2) and the indicated acceptor. Data available at http://doi.org/10.5281/zenodo.580169.