Figure 2 | Scientific Reports

Figure 2

From: Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2

Figure 2

Schematic overview of the fusion constructs used in this study. The differences in the amino acid sequence of the C-termini of the acceptor fluorescent proteins are depicted. The distance between the acceptor chromophore and its C-terminus is 158 amino acids for mKO2 and mKOκ, 163 amino acids for mOrange, mOrange2, mScarlet-I and mCherry, 164 amino acids for Clover, 166 amino acids for mNeonGreen, TagRFP-T and mKate2, 168 amino acids for mRuby2 and 171 amino acids for EGFP, SYFP2 and sREACh. The acceptors are followed by a small linker, which is the same for each construct, separating it from the donor mTurquoise2 (mTq2).

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