Figure 5
From: Identification and characterization of a novel Sso7d scaffold-based binder against Notch1
N9 decreases tumorsphere growth of patient derived cell line. (A) Patient derived colorectal cancer cell lines (PDCRCs) stained with commercial Notch1 antibody (red), N9 (green), and the nuclear stain DAPI (blue). Inset depicts magnified view of a representative colony. Note the similarity in staining pattern between commercial antibody and N9. (B) N9 (red line) decreases PDCRC cell viability. PDCRC cells were incubated with indicated concentration of binders for three days and cell viability was measured. (C) N9 decreases PDCRC tumorsphere formation. Single cell suspension of PDCRC cells were treated with 5 μM binders and 10 μM DAPT for five days and spheres bigger than 20μm were counted. (D) Representative images of PDCRC spheres. Inset depicts magnified view of a representative sphere. (*P < 0.05, **P < 0.01).
