Figure 6
From: Identification and characterization of a novel Sso7d scaffold-based binder against Notch1
Evaluation of N9 in immunostaining application. (A) HCT116 cells were stained with N9 (green), commercial Notch1 antibody (red) and DAPI (blue). Note the staining at cell-cell interfaces. (B) Fluorescence intensity profile of N9 and Notch1 staining of (A). Note the similarity in pattern of the green (N9) and red (Notch1) curves indicating co-localization of the signals. (C) HEK293 cells were transiently transfected with Notch1 and stained with N9 (green), commercial Notch1 antibody (red) and DAPI (blue). (C’) Magnified view of the inset in (C) showing staining of Notch1-transfected (arrowhead) and non-transfected (arrow) cells. Note that only Notch1 transfected cells (arrowhead) were stained with N9.
